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I've prior to now revealed one to POF binds so you're able to productive genetics to the the fresh next chromosome with a choice to have exons and this new amounts of POF joining so you're able to family genes correlate which have transcript profile (27). We thus wanted to determine whether there is certainly a love anywhere between new joining from POF and that away from RNA polymerase II (RNP2). To deal with this matter, i temperatures astonished Drosophila larvae to have forty five min during the 38°C and you may monitored the fresh new reassociation out-of POF and RNP2 so you can chromatin. Since are the case having RNP2, POF was also released from the normal chromatin address internet immediately after the warmth treat, nevertheless wasn't redistributed to help you temperature amaze-triggered family genes. One another POF in addition to RNP2 have been restored to their normal withdrawals towards chromosomes during a period of around 2 h ( Fig.
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